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Title: Mass spectrometry with direct supercritical fluid injection

Direct fluid injection mass spectrometry utilizes supercritical fluids for solvation and transfer of materials to a mass spectrometer chemical ionization (CI) source. Available data suggest that any material soluble in a supercritical fluid is transferred efficiently to the ionization region. Mass spectra are presented for mycotoxins of the trichothecene group obtained by use of supercritical carbon dioxide with isobutane as the CI reagent gas. Direct fluid injection MS/MS is also illustrated for major ions in the isobutane chemical ionization of T-2 toxin. The effect of pressure and temperature upon solubility in supercritical fluids is described and illustrated for diacetoxyscirpenol. A potential method is also demonstrated for ''on-line fractionation'' during MS analysis using pressure to control supercritical fluid solubility. Mass spectra are also presented for polar compounds, using supercritical ammonia, and the extension to complex mixtures is described. The fundamental basis and experimental requirements of the direct fluid injection process are discussed. 1 figure, 11 tables.
Authors:
;
Publication Date:
OSTI Identifier:
6972431
DOE Contract Number:
AC06-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Anal. Chem.; (United States); Journal Volume: 55:14
Research Org:
Pacific Northwest Lab., Richland, WA
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; AMINES; MASS SPECTRA; CHLORINATED AROMATIC HYDROCARBONS; COAL LIQUIDS; MASS SPECTROMETERS; EQUIPMENT INTERFACES; FLUID INJECTION PROCESSES; SUPERCRITICAL FLUID CHROMATOGRAPHY; TOXINS; 2-METHYLPROPANE; AMMONIA; CALIBRATION; CARBON DIOXIDE; CONDENSED AROMATICS; CRITICAL PRESSURE; CRITICAL TEMPERATURE; EXPERIMENTAL DATA; FEASIBILITY STUDIES; FLOW RATE; GASES; HIGH PRESSURE; HIGH TEMPERATURE; MEDIUM PRESSURE; MEDIUM TEMPERATURE; PRESSURE DEPENDENCE; QUALITATIVE CHEMICAL ANALYSIS; REAGENTS; SOLVATION; SOLVENT PROPERTIES; TEMPERATURE DEPENDENCE; TIME DEPENDENCE; ALKANES; ANTIGENS; AROMATICS; CARBON COMPOUNDS; CARBON OXIDES; CHALCOGENIDES; CHEMICAL ANALYSIS; CHROMATOGRAPHY; DATA; FLUIDS; HALOGENATED AROMATIC HYDROCARBONS; HYDRIDES; HYDROCARBONS; HYDROGEN COMPOUNDS; INFORMATION; LIQUIDS; MATERIALS; MEASURING INSTRUMENTS; NITROGEN COMPOUNDS; NITROGEN HYDRIDES; NUMERICAL DATA; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PHYSICAL PROPERTIES; SEPARATION PROCESSES; SPECTRA; SPECTROMETERS; THERMODYNAMIC PROPERTIES; TOXIC MATERIALS; TRANSITION TEMPERATURE 400104* -- Spectral Procedures-- (-1987)