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Title: Metabolism of circulating renin by liver and kidney of rats

Rat renal renin, highly purified and labeled with /sup 125/I, was intravenously given to conscious rats, to study the fate of the circulating renin. Rat antirenin antiserum was used to identify the labeled renin. The disappearance of (/sup 125/I)-renin from the plasma showed two exponential components and the metabolic clearance rate was 11.4 +/- 1.0 ml/min/kg. Both 70% hepatectomy and bilateral nephrectomy decreased the clearance rate by about 50%. (/sup 125/I)-renin accumulated mainly in the liver and kidney, and high performance liquid chromatography (HPLC) analysis indicated the degradation of (/sup 125/I)-renin by these organs. Biliary excretion of (/sup 125/I)-renin was negligible and urinary excretion accounted for 2% of the injected dose. Light- and electron-microscopic autoradiography indicated that (/sup 125/I)-renin is taken up mainly by Kupffer cells and proximal convoluted tubular cells in the liver and kidney, respectively, and thereafter distributes to the lysosomes. In conclusion, both the liver and kidney are responsible for the clearance of circulating renin.
Authors:
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Publication Date:
OSTI Identifier:
6924403
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Cardiovasc. Pharmacol.; (United States)
Research Org:
Osaka City Univ. Medical School (Japan)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; RENIN; METABOLISM; AUTORADIOGRAPHY; CLEARANCE; INTRAVENOUS INJECTION; IODINE 125; KIDNEYS; LABELLED COMPOUNDS; LIQUID COLUMN CHROMATOGRAPHY; LIVER; RATS; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; GLANDS; HYDROLASES; INJECTION; INTAKE; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; MAMMALS; NONSPECIFIC PEPTIDASES; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PEPTIDE HYDROLASES; RADIOISOTOPES; RODENTS; SEPARATION PROCESSES; VERTEBRATES 550501* -- Metabolism-- Tracer Techniques