Metabolism of circulating renin by liver and kidney of rats
Rat renal renin, highly purified and labeled with /sup 125/I, was intravenously given to conscious rats, to study the fate of the circulating renin. Rat antirenin antiserum was used to identify the labeled renin. The disappearance of (/sup 125/I)-renin from the plasma showed two exponential components and the metabolic clearance rate was 11.4 +/- 1.0 ml/min/kg. Both 70% hepatectomy and bilateral nephrectomy decreased the clearance rate by about 50%. (/sup 125/I)-renin accumulated mainly in the liver and kidney, and high performance liquid chromatography (HPLC) analysis indicated the degradation of (/sup 125/I)-renin by these organs. Biliary excretion of (/sup 125/I)-renin was negligible and urinary excretion accounted for 2% of the injected dose. Light- and electron-microscopic autoradiography indicated that (/sup 125/I)-renin is taken up mainly by Kupffer cells and proximal convoluted tubular cells in the liver and kidney, respectively, and thereafter distributes to the lysosomes. In conclusion, both the liver and kidney are responsible for the clearance of circulating renin.
- Research Organization:
- Osaka City Univ. Medical School (Japan)
- OSTI ID:
- 6924403
- Journal Information:
- J. Cardiovasc. Pharmacol.; (United States), Journal Name: J. Cardiovasc. Pharmacol.; (United States)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
RENIN
METABOLISM
AUTORADIOGRAPHY
CLEARANCE
INTRAVENOUS INJECTION
IODINE 125
KIDNEYS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
RATS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLANDS
HYDROLASES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
VERTEBRATES
550501* - Metabolism- Tracer Techniques