Poliovirus RNA synthesis in vitro: structural elements and antibody inhibition
The poliovirus RNA polymerase complex has been analyzed by immunoautoradiography using antibody probes derived from purified replicase (P3) region viral polypeptides. Antibody preparations made against the polio RNA polymerase, P3-4b, detected a previously unreported cellular protein that copurifies with the RNA polymerase. An IgG fraction purified from rabbit antiserum to polypeptide P3-2, a precursor fo the RNA polymerase, specifically inhibits poliovirus RNA synthesis in vitro. The authors have also immunoprecipitated a 60,000-dalton protein (P3-4a) with antiserum to protein P3-4b and have determined the precise genomic map position of this protein by automated Edman degradation. Protein P3-4a originates by cleavage of the RNA polymerase precursor at a glutamine-glucine amino acid pair not previously reported to be a viral cleavage site.
- Research Organization:
- State Univ. of New York, Stony Brook
- OSTI ID:
- 6917412
- Journal Information:
- Virology; (United States), Vol. 126
- Country of Publication:
- United States
- Language:
- English
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ANTIBODIES
INHIBITION
POLIO VIRUS
BIOSYNTHESIS
IN VITRO
IODINE 125
LABELLED COMPOUNDS
POLYMERASES
PROTEINS
RABBITS
RNA
SULFUR 35
TRACER TECHNIQUES
TRITIUM
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HYDROGEN ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
SULFUR ISOTOPES
SYNTHESIS
TRANSFERASES
VERTEBRATES
VIRUSES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques