Cooperation between the polyomavirus Middle-T-antigen gene and the human c-myc oncogene in a rat thyroid epithelial differentiated cell line: Model of in vitro progression
Two rat thyroid epithelial differentiated cell lines, PC CI 3 and PC myc, were infected with the polyoma murine leukemia virus (PyMLV) carrying the Middle-T-antigen gene of polyomavirus. After infection, both cell lines acquired the typical markers of neoplastic transformation; however, the PC myc cells showed a greater malignant phenotype. Furthermore, the thyroid differentiated functions were completely suppressed in PC myc cells transformed by PyMLV, whereas they were, at least partially, retained in PC CI 3 cells transformed by PyMLV, and in particular, thyroglobulin synthesis and secretion were not affected at all. Since no differences in the expression of the middle-T-antigen gene were observed in the two PyMLV-transformed cell lines, the different properties shown by these two infected cell lines must be ascribed to the expression of the c-myc oncogene.
- Research Organization:
- Centro di Endocrinologia ed Oncologia Sperimentale C.N.R., Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolta di Medicina e Chirurgia, Universita di Napoli, via Pansini 5, 80131 Naples (IT)
- OSTI ID:
- 6902970
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cooperation of c-raf-1 and c-myc protooncogenes in the neoplastic transformation of simian virus 40 large tumor antigen-immortalized human bronchial epithelial cells
Transformation by viral and cellular oncogenes of a mouse BALB/3T3 cell mutant resistant to transformation by chemical carcinogens
Related Subjects
ONCOGENES
MOLECULAR BIOLOGY
ONCOGENIC TRANSFORMATIONS
POLYOMA VIRUS
GENETIC MAPPING
THYROID CELLS
ANTIGENS
BIOLOGICAL MODELS
BIOLOGICAL PATHWAYS
CELL DIFFERENTIATION
EPITHELIUM
GENE REPRESSORS
IN VITRO
LYMPHOCYTES
RATS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
GENES
LEUKOCYTES
MAMMALS
MAPPING
MATERIALS
MICROORGANISMS
NUCLEOPROTEINS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
VIRUSES
550200* - Biochemistry
550400 - Genetics