Hydration of DNA by tritiated water and isotope distribution: a study by /sup 1/H, /sup 2/H, and /sup 3/H NMR spectroscopy
The hydration layer of DNA (0.75%) in tritiated water represents 3.5% of solvent /sup 3/HHO. The combined effects of temperature (-6 to -40/sup 0/C) and H/sub 2/O//sup 2/H/sub 2/O solvent composition on the spin-lattice relaxation times of water protons and deuterons suggest selective distribution of isotopes in the hydration layer. The ''hydration isotope'' effect and the localization of tritiated water molecules in the hydration layer of DNA have important implications in describing the radiobiological effects of tritiated water because the initial molecular damage caused by /sup 3/HHO (internal radiation source) localizes close to /sup 3/H due to the short range and low energy of /sup 3/H ..beta.. rays.
- Research Organization:
- Institute d'Hygiene et d'Epidemiologie, Brussels, Belgium
- OSTI ID:
- 6842416
- Journal Information:
- Radiat. Res.; (United States), Vol. 90:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL RADIATION EFFECTS
HYDRATION
SPIN-LATTICE RELAXATION
TEMPERATURE DEPENDENCE
TRITIUM COMPOUNDS
NMR SPECTRA
TRITIUM OXIDES
BIOLOGICAL LOCALIZATION
DEUTERIUM
HYDROGEN 1
RADIOSENSITIVITY EFFECTS
BIOLOGICAL EFFECTS
CHALCOGENIDES
HYDROGEN ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
RADIATION EFFECTS
RELAXATION
SOLVATION
SPECTRA
STABLE ISOTOPES
560114* - Radiation Effects on Biochemicals- In Animals- (-1987)
560122 - Radiation Effects on Cells- Internal Source- (-1987)