Effects of. gamma. irradiation on the hydration characteristics of DNA and polynucleotides. I. An NMR study of frozen H/sub 2/O and D/sub 2/O solutions
Conference
·
· Radiat. Res.; (United States)
OSTI ID:7338255
- Univ. Libre, Brussels
Water proton and deuteron resonance spectra have been studied in frozen solutions of DNA, poly(A), poly(C), poly(U), poly(A + U) and poly(A + 2U) at different temperatures between -5 and -40/sup 0/C. Hydration water molecules exhibit high proton and molecular mobility in frozen solutions and are responsible for the observed spectra. The ..gamma.. irradiation of solutions at 0 and -80/sup 0/C results in striking changes in the proton spectra and no change in the deuteron spectra. These results suggest that proton exchange is an important factor in affecting the proton linewidth and the proton relaxation mechanism. The changes in molecular structure (either by ..gamma.. irradiation or molecular association) influence the hydration water in a characteristic manner. The effects of irradiation at different temperatures (0, -80, and -196/sup 0/C) suggest that hydration water makes a distinct contribution to the overall radical attack on the DNA molecule from the water medium.
- OSTI ID:
- 7338255
- Conference Information:
- Journal Name: Radiat. Res.; (United States) Journal Volume: 68:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHARGED PARTICLES
DEUTERONS
DISPERSIONS
DNA
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
FERMIONS
GAMMA RADIATION
HADRONS
HYDRATION
HYDROGEN COMPOUNDS
IONIZING RADIATIONS
MAGNETIC RESONANCE
MIXTURES
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
NUCLEOTIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTONS
RADIATION EFFECTS
RADIATIONS
RADICALS
RESONANCE
SOLUTIONS
SOLVATION
SPECTRA
WATER
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHARGED PARTICLES
DEUTERONS
DISPERSIONS
DNA
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
FERMIONS
GAMMA RADIATION
HADRONS
HYDRATION
HYDROGEN COMPOUNDS
IONIZING RADIATIONS
MAGNETIC RESONANCE
MIXTURES
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
NUCLEOTIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTONS
RADIATION EFFECTS
RADIATIONS
RADICALS
RESONANCE
SOLUTIONS
SOLVATION
SPECTRA
WATER