Hydration of DNA by tritiated water and isotope distribution: a study by /sup 1/H, /sup 2/H, and /sup 3/H NMR spectroscopy
Journal Article
·
· Radiat. Res.; (United States)
OSTI ID:6842416
The hydration layer of DNA (0.75%) in tritiated water represents 3.5% of solvent /sup 3/HHO. The combined effects of temperature (-6 to -40/sup 0/C) and H/sub 2/O//sup 2/H/sub 2/O solvent composition on the spin-lattice relaxation times of water protons and deuterons suggest selective distribution of isotopes in the hydration layer. The ''hydration isotope'' effect and the localization of tritiated water molecules in the hydration layer of DNA have important implications in describing the radiobiological effects of tritiated water because the initial molecular damage caused by /sup 3/HHO (internal radiation source) localizes close to /sup 3/H due to the short range and low energy of /sup 3/H ..beta.. rays.
- Research Organization:
- Institute d'Hygiene et d'Epidemiologie, Brussels, Belgium
- OSTI ID:
- 6842416
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 90:3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Fri Dec 30 23:00:00 EST 1994
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OSTI ID:381092
Related Subjects
560114* -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
560122 -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL LOCALIZATION
BIOLOGICAL RADIATION EFFECTS
CHALCOGENIDES
DEUTERIUM
DNA
HYDRATION
HYDROGEN 1
HYDROGEN ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NMR SPECTRA
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIOSENSITIVITY EFFECTS
RELAXATION
SOLVATION
SPECTRA
SPIN-LATTICE RELAXATION
STABLE ISOTOPES
TEMPERATURE DEPENDENCE
TRITIUM COMPOUNDS
TRITIUM OXIDES
560122 -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL LOCALIZATION
BIOLOGICAL RADIATION EFFECTS
CHALCOGENIDES
DEUTERIUM
DNA
HYDRATION
HYDROGEN 1
HYDROGEN ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NMR SPECTRA
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIOSENSITIVITY EFFECTS
RELAXATION
SOLVATION
SPECTRA
SPIN-LATTICE RELAXATION
STABLE ISOTOPES
TEMPERATURE DEPENDENCE
TRITIUM COMPOUNDS
TRITIUM OXIDES