Endotoxin increases pulmonary vascular protein permeability in the dog

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · J. Appl. Physiol.; (United States)

OSTI ID:6842065

Welsh, C H; Dauber, I M; Weil, J V

Endotoxin increases pulmonary vascular permeability consistently in some species but fails to reliably cause injury in the dog. We wondered whether this phenomenon depended on the method of injury assessment, as others have relied on edema measurement; we quantified injury by monitoring the rate of extravascular protein accumulation. /sup 113m/In-labeled protein and /sup 99m/Tc-labeled erythrocytes were injected into anesthetized dogs and monitored by an externally placed lung probe. A protein leak index, the rate of extravascular protein accumulation, was derived from the rate of increase in lung protein counts corrected for changes in intravascular protein activity. After administration of Salmonella enteriditis endotoxin (4 micrograms/kg), the protein leak index was elevated 2.5-fold (41.1 +/- 4.6 X 10(-4) min-1) compared with control (16.0 +/- 2.8 X 10(-4) min-1). In contrast, wet-to-dry weight ratios failed to increase after endotoxin (4.6 +/- 0.8 vs. control values of 4.2 +/- 0.5 g/g dry bloodless lung). However, we observed that endotoxin increased lung dry weight (per unit body weight), which may have attenuated the change in wet-to-dry weight ratios. To determine whether low microvascular pressures following endotoxin attenuated edema formation, we increased pulmonary arterial wedge pressures in five dogs by saline infusion, which caused an increase in wet-to-dry weight ratios following endotoxin but no change in the five controls. We conclude that low dose endotoxin causes pulmonary vascular protein leak in the dog while edema formation is minimal or absent.

• Research Organization: Univ. of Colorado Health Sciences Center, Denver
• OSTI ID: 6842065
• Journal Information: J. Appl. Physiol.; (United States), Vol. 4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDOTOXINS
BIOLOGICAL EFFECTS
LUNGS
PERMEABILITY
DOGS
EDEMA
ERYTHROCYTES
INDIUM 113
LABELLED COMPOUNDS
PROTEINS
TECHNETIUM 99
TRACER TECHNIQUES
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
HOURS LIVING RADIOISOTOPES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
RADIOISOTOPES
RESPIRATORY SYSTEM
STABLE ISOTOPES
SYMPTOMS
TECHNETIUM ISOTOPES
TOXIC MATERIALS
TOXINS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
551001* - Physiological Systems- Tracer Techniques