5-Hydroxytryptamine uptake in oxygen radical-mediated acute lung injury

Journal Article · Wed Feb 01 04:00:00 EST 1989 · Am. Rev. Respir. Dis.; (United States)

DOI:https://doi.org/10.1164/ajrccm/139.2.382· OSTI ID:6265558

Vigneswaran, W T; Stanbrook, H S; Doctor, R; McCormick, S; Schwappach, J; Johnston, M R

Pulmonary endothelial cell function (ECF) studies have been shown to be a sensitive indicator of chronic lung injury. We attempted to correlate changes in 5-hydroxytryptamine (5HT) uptake with an acute oxygen radical-mediated lung injury in dogs. Beta-d glucose/glucose oxidase was injected intravenously in an experimental group (n = 10), while the control group (n = 5) received saline. 5HT uptake, measured using a multiple indicator dilution technique before and 20 min after injection, was calculated for both the percent total uptake and the peak extraction ratio of 5HT during a single passage through the lung. The mean pulmonary and systemic arterial pressures (PAP, SAP), total pulmonary resistance (TPR), extravascular lung water (EVLW), and wet-to-dry weight ratios were also determined. The experimental group showed an acute rise in PAP and TPR and a fall in SAP after the injection, all returning to normal by 20 min; total 5HT uptake fell from 81 +/- 2.3% to 47 +/- 6.5% (p = 0.0002) as did the peak extraction ratio from 0.87 +/- 0.013 to 0.44 +/- 0.066 (p = 0.0001). No change in 5HT uptake was observed in the control group. EVLW did not change in either group, but wet-to-dry weight ratio was elevated in the experimental group (5.21 +/- 0.12 versus 4.73 +/- 0.06, p less than 0.01). ECF studies of 5HT uptake appear to be a sensitive indicator of acute lung injury in this large-animal, oxygen radical-induced injury model.

• Research Organization: Univ. of Colorado School of Medicine, Denver (USA)
• OSTI ID: 6265558
• Journal Information: Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 139:2; ISSN ARDSB
• Country of Publication: United States
• Language: English

Related Subjects

550901 -- Pathology-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
AMINES
ANIMAL TISSUES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
BODY
CARBOHYDRATES
DISEASES
DOGS
DRUGS
ELEMENTS
ENDOTHELIUM
ENZYMES
EXTRACELLULAR SPACE
GLUCOSE
HETEROCYCLIC COMPOUNDS
HEXOSES
HYDROXY COMPOUNDS
INDOLES
INJURIES
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
LUNGS
MAMMALS
MONOSACCHARIDES
NEUROREGULATORS
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGEN
PYRROLES
RADICALS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
SACCHARIDES
SEROTONIN
SPACE
SYMPATHOMIMETICS
TISSUES
TRACER TECHNIQUES
TRYPTAMINES
UPTAKE
VERTEBRATES