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Title: Mechanisms of poliovirus inactivation by the direct and indirect effects of ionizing radiation

This study was designed to measure the effects of ionizing radiation on poliovirus particles when given under conditions where either direct (in broth) or indirect (in water) effects were predominant. Under direct conditions, inactivation of poliovirus was found to be due primarily to RNA damage, although capsid damage could account for about one-third of the viral inactivation. RNA damage did not appear to be due to strand breakage and therefore was probably caused primarily by base damage or crosslink formation. Capsid damage under direct irradiation conditions did not result in significant alterations of either the sedimentation coefficients or the isoelectric points of the poliovirus particles or detectable modification of the sizes of the viral proteins. It did, however, cause loss of availability to bind to host cells. Under indirect conditions no more than 25% of viral inactivation appeared to be due to RNA damage. However, the sedimentation coefficients and isoelectric points of the viral particles were greatly altered, and their abilities to bind to cells were lost at about three-fourths the rate of loss of infectivity. Capsid damage in this case did result in changes in the sizes of capsid proteins. Therefore, the majority of the radiation inactivation under indirectmore » conditions appeared to be due to protein damage.« less
Authors:
Publication Date:
OSTI Identifier:
6817801
DOE Contract Number:
E(29-2)-3536
Resource Type:
Journal Article
Resource Relation:
Journal Name: Radiat. Res.; (United States); Journal Volume: 83:2
Research Org:
Sandia Lbs., Albuquerque, NM
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; POLIO VIRUS; BIOLOGICAL RADIATION EFFECTS; INACTIVATION; RNA; CARBON 14 COMPOUNDS; COBALT 60; CROSS-LINKING; ELECTROPHORESIS; EXPERIMENTAL DATA; HELA CELLS; IONIZING RADIATIONS; NUCLEOSIDES; PROTEINS; RADIATION DOSES; STRAND BREAKS; SURVIVAL CURVES; TRITIUM COMPOUNDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; CHEMICAL REACTIONS; COBALT ISOTOPES; DATA; DOSES; INFORMATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LABELLED COMPOUNDS; MICROORGANISMS; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDES; NUMERICAL DATA; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PARASITES; POLYMERIZATION; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RIBOSIDES; VIRUSES; YEARS LIVING RADIOISOTOPES 560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987); 550701 -- Microbiology-- Tracer Techniques