Effect of acute ethanol on beta-endorphin secretion from rat fetal hypothalamic neurons in primary cultures
- Washington State Univ., Pullman (USA)
To characterize the effect of ethanol on the hypothalamic {beta}-endorphin-containing neurons, rat fetal hypothalamic neurons were maintained in primary culture, and the secretion of {beta}-endorphin ({beta}-EP) was determined after ethanol challenges. Constant exposure to ethanol at doses of 6-50 mM produced a dose-dependent increase in basal secretion of {beta}-EP from these cultured cells. These doses of ethanol did not produce any significant effect on cell viability, DNA or protein content. The stimulated secretion of {beta}-EP following constant ethanol exposure is short-lasting. However, intermittent ethanol exposures maintained the ethanol stimulatory action on {beta}-EP secretion for a longer time. The magnitude of the {beta}-EP response to 50 mM ethanol is similar to that of the {beta}-EP response to 56 mM of potassium. Ethanol-stimulated {beta}-EP secretion required extracellular calcium and was blocked by a calcium channel blocker; a sodium channel blocker did not affect ethanol-stimulated secretion. These results suggest that the neuron culture system is a useful model for studying the cellular mechanisms involved in the ethanol-regulated hypothalamic opioid secretion.
- OSTI ID:
- 6788698
- Journal Information:
- Life Sciences; (USA), Vol. 47:9; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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ENDORPHINS
SECRETION
ETHANOL
BIOLOGICAL EFFECTS
ACUTE EXPOSURE
CALCIUM
CELL CULTURES
DNA
DOSE-RESPONSE RELATIONSHIPS
FETUSES
HYPOTHALAMUS
METABOLISM
NERVE CELLS
PORINS
RATS
ALCOHOLS
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DRUGS
ELEMENTS
HYDROXY COMPOUNDS
MAMMALS
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METALS
NERVOUS SYSTEM
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NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology