beta. -endorphin modulation of mitogen-stimulated calcium uptake by rat thymocytes

Journal Article · Mon Oct 19 00:00:00 EDT 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90750-8· OSTI ID:6080773

Hemmick, L M; Bidlack, J M

Lymphocytes stimulated by mitogens or antigens exhibit an enhanced calcium uptake early in the proliferation or activation response. Modulation of this calcium uptake results in alterations of proliferation and immunocompetence. ..beta..-endorphin and other opioids affect several parameters of lymphocyte competence. Limited data are available concerning the mechanism(s) of these effects. This study examines whether a possible opioid mechanism is the modification of the early calcium influx into stimulated lymphocytes. The time course of both concanavalin A (Con A) and phytohemagglutinin (PHA)-stimulated /sup 45/Ca/sup 2 +/ uptake into thymocytes was characterized to determine the optimal time for testing the effects of opioids. BETA-Endorphin 1-31 significantly enhanced Con A-stimulated /sup 45/Ca/sup 2 +/ uptake into rat thymocytes. This peptide had no significant effect on PHA-simulated /sup 45/Ca/sup 2 +/ uptake or on basal thymocyte /sup 45/Ca/sup 2 +/ flux. The ..beta../sub h/-endorphin stimulatory effect was titratable in the range of 0.1 nM to 10 ..mu..M. Naloxone did not reverse the enhancement. Met-enkephalinamide and other opioid agonists did not duplicate the stimulatory effect. Thus, the ..beta../sub h/-endorphin 1-31 enhancement of Con A-stimulated /sup 45/Ca/sup 2 +/ uptake by rat thymocytes does not operate via classical opioid receptor mechanisms. ..beta../sub h/-endorphin 1-31 appears to be acting on a subset of T cells that are responsive to Con A but not to PHA. 30 references, 4 figures, 1 table.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6080773
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 41:16; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARBOHYDRATES
CELL PROLIFERATION
CONCANAVALIN
CONNECTIVE TISSUE CELLS
DATA
DAYS LIVING RADIOISOTOPES
DRUGS
ENDORPHINS
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
FUNCTIONS
HEMAGGLUTININS
INFORMATION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LECTINS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MITOGENS
MUCOPROTEINS
NEUROREGULATORS
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SOMATIC CELLS
THYMOCYTES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES