Effect of acute ethanol on beta-endorphin secretion from rat fetal hypothalamic neurons in primary cultures
- Washington State Univ., Pullman (USA)
To characterize the effect of ethanol on the hypothalamic {beta}-endorphin-containing neurons, rat fetal hypothalamic neurons were maintained in primary culture, and the secretion of {beta}-endorphin ({beta}-EP) was determined after ethanol challenges. Constant exposure to ethanol at doses of 6-50 mM produced a dose-dependent increase in basal secretion of {beta}-EP from these cultured cells. These doses of ethanol did not produce any significant effect on cell viability, DNA or protein content. The stimulated secretion of {beta}-EP following constant ethanol exposure is short-lasting. However, intermittent ethanol exposures maintained the ethanol stimulatory action on {beta}-EP secretion for a longer time. The magnitude of the {beta}-EP response to 50 mM ethanol is similar to that of the {beta}-EP response to 56 mM of potassium. Ethanol-stimulated {beta}-EP secretion required extracellular calcium and was blocked by a calcium channel blocker; a sodium channel blocker did not affect ethanol-stimulated secretion. These results suggest that the neuron culture system is a useful model for studying the cellular mechanisms involved in the ethanol-regulated hypothalamic opioid secretion.
- OSTI ID:
- 6788698
- Journal Information:
- Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 47:9; ISSN 0024-3205; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ALCOHOLS
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BODY
BRAIN
CALCIUM
CELL CULTURES
CENTRAL NERVOUS SYSTEM
DNA
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELEMENTS
ENDORPHINS
ETHANOL
FETUSES
HYDROXY COMPOUNDS
HYPOTHALAMUS
MAMMALS
MEMBRANE PROTEINS
METABOLISM
METALS
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PORINS
PROTEINS
RATS
RODENTS
SECRETION
SOMATIC CELLS
VERTEBRATES