Radiometric-microbiologic assay of niacin using Kloeckera brevis: analysis of human blood and food

Journal Article · Thu Dec 01 00:00:00 EST 1983 · J. Nutr.; (United States)

OSTI ID:6630908

Guilarte, T R; Pravlik, K

Kloeckera brevis, a yeast, was used as the test organism for the development of a radiometric-microbiologic (RMA) assay for niacin. The assay was determined to be sensitive to the 2 ng niacin per vial level and specific for the biologically active forms of this vitamin. The method was shown to be simple, accurate, and precise in the analysis of niacin in human blood and food. The application of the radiometric technique eliminates some of the problems encountered with conventional turbidimetric-microbiologic assay.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• OSTI ID: 6630908
• Journal Information: J. Nutr.; (United States), Vol. 113:12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD
CHEMICAL ANALYSIS
FOOD
NICOTINIC ACID
BIOASSAY
RADIOMETRIC ANALYSIS
YEASTS
GROWTH
DIET
MAN
SAMPLING
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOXYLIC ACIDS
FUNGI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
MATERIALS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PRIMATES
PYRIDINES
QUANTITATIVE CHEMICAL ANALYSIS
VERTEBRATES
VITAMIN B GROUP
VITAMINS
550201* - Biochemistry- Tracer Techniques