Radiometric--microbiologic assay of vitamin B-6: application to food analysis

Journal Article · Sun Nov 01 04:00:00 EST 1981 · J. Nutr.; (United States)

OSTI ID:5503750

Guilarte, T R; Shane, B; McIntyre, P A

A radiometric microbiologic assay for vitamin B-6 was applied to food analysis. The method was shown to be specific, reproducible and simpler than the standard turbidimetric microbiologic technique. The analysis of seven commercially available breakfast cereals was compared to a high performance liquid chromatography method. Three out of the seven cereals agreed when assayed with both methods (P greater than 0.1). Four cereals, however, differed in value considerably (P less than 0.05). Further studies are required to determine whether these differences were due to different extraction procedures used. The study showed that the new radiometric-microbiologic method can be used to measure total vitamin B-6 or, combined with a column separation procedure, to analyze for specific forms of the vitamin.

• Research Organization: Department of Environmental Health Sciences, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD
• OSTI ID: 5503750
• Journal Information: J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 111:11; ISSN JONUA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
553001 -- Agriculture & Food Technology-- Tracer Techniques-- (-1987)
60 APPLIED LIFE SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AZINES
CHEMICAL COMPOSITION
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
FOOD
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
PYRIDOXINE
RADIOASSAY
SEPARATION PROCESSES
VITAMIN B GROUP
VITAMINS