Radiometric microbiologic assay for the biologically active forms of niacin

Journal Article · Tue May 01 04:00:00 EDT 1979 · J. Nucl. Med.; (United States)

OSTI ID:6296789

Kertcher, J A; Guilarte, T R; Chen, M F; Rider, A A; McIntyre, P A

A radiometric microbiologic assay has been developed for the determination of niacin in biologic fluids. Lactobacillus plantarum produced /sup 14/CO/sub 2/ from L-(U-/sup 14/C) malic acid in quantities proportional to the amount of niacin present. The assay is specific for the biologically active forms of niacin in humans. Thirty normal hemolysates were analyzed and the values ranged from 13.0 to 17.8 ..mu..g niacin/ml RBC (mean = 15.27 +- 1.33 s.d.). Good recovery and reproducibility studies were obtained with this assay. On thirty blood samples, correlation was excellent between the radiometric and the conventional turbidimetric assays.

• Research Organization: Johns Hopkins Medical Institutions, Baltimore, MD
• OSTI ID: 6296789
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 20:5; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
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550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AZINES
BACTERIA
BIOASSAY
BLOOD SERUM
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
LABELLED COMPOUNDS
LACTOBACILLUS
MALIC ACID
MAMMALS
MAN
METABOLISM
MICROORGANISMS
NICOTINIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PRIMATES
PYRIDINES
RADIOASSAY
VERTEBRATES
VITAMIN B GROUP
VITAMINS