Radiometric-microbiologic assay of niacin using Kloeckera brevis: analysis of human blood and food

Journal Article · Thu Dec 01 04:00:00 EST 1983 · J. Nutr.; (United States)

OSTI ID:6630908

Guilarte, T R; Pravlik, K

Kloeckera brevis, a yeast, was used as the test organism for the development of a radiometric-microbiologic (RMA) assay for niacin. The assay was determined to be sensitive to the 2 ng niacin per vial level and specific for the biologically active forms of this vitamin. The method was shown to be simple, accurate, and precise in the analysis of niacin in human blood and food. The application of the radiometric technique eliminates some of the problems encountered with conventional turbidimetric-microbiologic assay.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• OSTI ID: 6630908
• Journal Information: J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 113:12; ISSN JONUA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AZINES
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
DIET
FOOD
FUNGI
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
MAN
MATERIALS
MICROORGANISMS
NICOTINIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PRIMATES
PYRIDINES
QUANTITATIVE CHEMICAL ANALYSIS
RADIOMETRIC ANALYSIS
SAMPLING
VERTEBRATES
VITAMIN B GROUP
VITAMINS
YEASTS