Effects of GA sub 4 on gene expression in isolated nuclei

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6611099

Sechley, K A; Srivastava, L M ^[1]

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1. Simon Fraser Univ., Burnaby, British Columbia (Canada)

GA{sub 4} is active in promoting elongation in cucumber (Cucuminus sativus) hypocotyls. Nuclei, isolated from the apical 1cm portion of the hypocotyl and purified on Percoll step gradients increased transcription (({sup 3}H)UTP incorporation into TCA precipitable products) in the presence of GA{sub 4} compared to untreated nuclei. This response was not observed in nuclei isolated from basal (nontarget) regions of the hypocotyl. Enhanced transcription in the presence of GA{sub 4} appears to be affected by proteinaceous factors which can be washed out of the nuclei. The GA{sub 4} induced response of cucumber hypocotyls is temperature sensitive; nuclei isolated from plants grown at 34C do not show the above GA{sub 4}-induced transcriptional enhancement. Comparison of 2-D flurograms of in vitro translation products synthesized from transcripts obtained from intact plants and isolated nuclei in the presence or absence of GA{sub 4} is currently in progress.

• OSTI ID: 6611099
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CUCUMBERS
GENE REGULATION
GIBBERELLIC ACID
BIOLOGICAL FUNCTIONS
CELL NUCLEI
TRACER TECHNIQUES
TRANSCRIPTION
TRITIUM COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
ESTERS
FOOD
FUNCTIONS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LACTONES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
VEGETABLES
550201* - Biochemistry- Tracer Techniques