Competition for in vitro (/sup 3/H)gibberellin A/sub 4/ binding in cucumber by gibberellins and their derivatives. [Cucumis sativus L. cv National Pickling]
Journal Article
·
· Plant Physiol.; (United States)
OSTI ID:6942807
The gibberellin (GA) binding properties of a cytosol fraction from hypocotyls of cucumber (Cucumis sativus L. cv National Pickling) were examined using a DEAE filter paper assay, (/sup 3/H)GA/sub 4/, and over 20 GAs, GA derivatives and other growth regulators. The results demonstrate structural specificity of the binding protein for ..gamma..-lactonic C-19 GAs with a 3 ..beta..-hydroxyl and a C-6 carboxyl group. Additional hydroxylations of the A, C, or D ring of the ent-gibberellane skeleton and methylation of the C-6 carboxyl impede or abolish binding affinity. Bioassay data are generally supported by the in vitro results but significantly GA/sub 9/ and GA/sub 36/, both considered to be precursors of GA/sub 4/ in cucumber, show no affinity for the binding protein. The results are discussed in relation to the active site of the putative GA/sub 4/ receptor in cucumber.
- Research Organization:
- Simon Fraser Univ., Burnaby, British Columbia
- OSTI ID:
- 6942807
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 79:4; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
BIOASSAY
CARBOXYLIC ACIDS
CHEMICAL BONDS
CHEMICAL REACTIONS
CPB
CUCUMBERS
DATA
ESTERS
EXPERIMENTAL DATA
FOOD
GIBBERELLIC ACID
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXYLATION
IN VITRO
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTONES
LEAVES
METHYLATION
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANT GROWTH REGULATORS
PLANTS
PRECURSOR
PROTEINS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEGETABLES
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
BIOASSAY
CARBOXYLIC ACIDS
CHEMICAL BONDS
CHEMICAL REACTIONS
CPB
CUCUMBERS
DATA
ESTERS
EXPERIMENTAL DATA
FOOD
GIBBERELLIC ACID
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXYLATION
IN VITRO
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTONES
LEAVES
METHYLATION
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANT GROWTH REGULATORS
PLANTS
PRECURSOR
PROTEINS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEGETABLES