Solutes in the free space of growing stem tissues

Journal Article · Sat Jan 01 00:00:00 EST 1983 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.72.2.326· OSTI ID:5910281

Cosgrove, D J; Cleland, R E

The concentration of osmotically active solutes in the cell wall free space of young stem tissues was studied using a variety of extraction methods. When the intercellular air spaces of etiolated pea (Pisum sativum L.) internodes were perfused with distilled H/sub 2/O, the resulting solution contained a solute concentration of about 70 milliosmoles per kilogram. A second procedure involving vacuum infiltration of segments followed by centrifugation to collect the free space solution gave similar results. Apical stem segments yielded free space extracts about twice as concentrated as those from basal portions of the stem. After correcting for dilution of the free space solution by the infiltrated water, the osmotic pressure of the undiluted free space in pea stem tissue was estimated to be 2.9 bars for apical segments, 1.8 bars for basal regions. These values may be somewhat overestimated due to solute efflux from intracellular pools during the extraction procedure. Similar results were obtained for stem regions of etiolated soybean (Glycine max (L.) Merr.) and cucumber (Cucumis sativus L.) seedlings. From measurements of the electrical conductivity and refractive index of free space extracts before and after ashing, it appears that 25% of the solutes are inorganic electrolytes and 75% are organic nonelectrolytes with an average size similar to that of glucose. A significant osmotic pressure in the wall space offers an explanation for the frequent observation that nontranspiring plants have negative water potentials. Calculations of hydraulic resistance from water potential data must take into account solutes in the free space, else apparent, but unreal, changes in resistance may be calculated. 27 references, 4 tables.

• Research Organization: Univ. of Washington, Seattle, WA (United States)
• DOE Contract Number: AT06-70ER73019
• OSTI ID: 5910281
• Journal Information: Plant Physiol.; (United States), Vol. 72
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CUCUMBERS
METABOLISM
GLYCINE HISPIDA
PISUM
PLANT CELLS
MEMBRANE TRANSPORT
CELL WALL
COMPARATIVE EVALUATIONS
EXPERIMENTAL DATA
MASS TRANSFER
OSMOSIS
PLANT GROWTH
SEEDLINGS
CELL CONSTITUENTS
DATA
DIFFUSION
FOOD
GROWTH
INFORMATION
LEGUMINOSAE
NUMERICAL DATA
PLANTS
VEGETABLES
550500* - Metabolism