Transforming growth factor type. beta. can act as a potent competence factor for AKR-2B cells

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(87)90388-0· OSTI ID:6529464

Goustin, A S; Nuttall, G A; Leof, E B; Ranganathan, G; Moses, H L ^[1]

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1. Mayo Clinic and Foundation, Rochester, MN (USA)

Transforming growth factor type {beta} (TGF{beta}) is a pleiotropic regulator of cell growth with specific high-affinity cell-surface receptors on a large number of cells; its mechanism of action, however, is poorly defined. In this report, the authors utilized the mouse fibroblast line AKR-2B to explore the question of the temporal requirements during the cell cycle in regard to both the growth inhibitory and the growth stimulatory action of TGF{beta}. The results indicate that AKR-2B cells are most sensitive to the inhibitory action of TGF{beta} during early to mid-G{sub 1}. In addition, TGF{beta} need be present only briefly in order to exert its inhibitory effect on EGF-induced DNA synthesis. Likewise, the stimulatory effect of TGF{beta} in the absence of EGF requires only an equally brief exposure to TGF{beta}. Use of homogeneous {sup 125}I-labeled TGF{beta} in a cell-binding assay demonstrates that TGF{beta} bound to cell-surface receptors can readily exchange into the culture medium, helping to rule out the possibility that persistent receptor-bound TGF{beta} is the source of a continuous stimulus. The data indicate that TGF{beta} exposure induces a stable state in the cell similar to but distinct from the state of competence induced by platelet-derived growth factor (PDGF).

• OSTI ID: 6529464
• Journal Information: Experimental Cell Research; (USA), Vol. 172:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

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