Growth factor regulation of sugar uptake in cultured cells
Mouse EGF stimulates the uptake of 2-deoxygluclose (dGIc), a non-metabolized glucose analogue, into cultured mouse 3T3 fibroblasts (Clone 1) 2 to 4 fold, and EGF dependent Balb/MK-1 epidermal kerotinocytes, 5 to 8 fold. Initial stimulation is detected at 15 minutes. Maximal effects are seen at 2 hours with 10 ng/ml EGF. Binding of /sup 125/I-labeled EGF to cells is rapid and complete by 2 hours at 37/sup 0/C. Antibodies which specifically inhibit /sup 125/I-labeled EGF binding to cells inhibit EGF stimulation as much as 85%. Human platelet derived TGF-..beta.. stimulates dGlc uptake up to 5 fold. Maximum effects are seen with 1 ng/ml TGF-..beta.. within 2 hours and stimulation is detected 30 minutes after exposure to 0.1 ng/ml, the minimum effective concentration. TGF-..beta.., like EGF, stimulates sugar transport into Balb/MK-1 cells without additional factors. However, neither stimulates uptake in a 3T3 variant, NR-6, which is EGF-receptor negative. The co-addition of EGF and/or PDGF enhances TGF-..beta.. stimulation. Binding of /sup 125/I-labeled TGF-..beta.. is nearly complete by 1 hour at 37/sup 0/C, but continues to increase for as long as 4 hours after addition. Antibodies which inhibit EGF binding have no effect on TGF-..beta.. binding, but they block TGF-..beta.. stimulation of hexose uptake. It is concluded from these results that the TGF-..beta.. receptor is distinct from the EGF receptor, and that although TGF-..beta.. stimulation of dGLc uptake does not require exogenously added EGF, it does require an active or available EGF receptor kinase system.
- Research Organization:
- Vanderbilt Univ., Nashville, TN (USA)
- OSTI ID:
- 7108217
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Insulin-like synergistic stimulation of DNA synthesis in Swiss 3T3 cells by the BSC-1 cell-derived growth inhibitor related to transforming growth factor type. beta
Regulation of transferrin receptor expression at the cell surface by insulin-like growth factors, epidermal growth factor and platelet-derived growth factor
Related Subjects
GLUCOSE
UPTAKE
GROWTH
STIMULATION
IODINE 125
TRACER TECHNIQUES
PROTEINS
CONFIGURATION INTERACTION
RECEPTORS
BLOOD PLATELETS
CELL CULTURES
FIBROBLASTS
GANGLIOSIDES
MICE
PHOSPHOTRANSFERASES
ALDEHYDES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HEXOSES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
550301* - Cytology- Tracer Techniques