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Stimulation of glucose uptake by transforming growth factor. beta. : evidence for the requirement of epidermal growth factor-receptor activation

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
;
Transforming growth factor ..beta.. (TGF-..beta..), derived from human platelets, stimulates the uptake of 2-deoxyglucose by cultured cell monolayers 2- to 4-fold. Stimulation can be detected as early as 30 min with as little as 0.1 ng of TGF-..beta.. per ml and maximal effects can be obtained at 2 hr with 1 ng of the growth factor per ml. TGF-..beta..-induced stimulation of sugar uptake is enhanced by the co-addition of platelet-derived growth factor (10 ng/ml) or epidermal growth factor (EGF, 1 ng/ml). The NR-6 variant of mouse 3T3 cells, which lack EGF receptors, is not stimulated by TGF-..beta... Antisera to EGF receptors that block /sup 125/I-labeled EGF binding also inhibit TGF-..beta.. stimulation of 2-deoxyglucose uptake, although /sup 125/I-labeled TGF-..beta.. binding remains unimpaired. In contrast, antisera to the EGF receptor, which do not block EGF binding, have no measurable effect on the TGF-..beta..-stimulated uptake of 2-deoxyglucose. The authors confirm that the receptor for TGF-..beta.. is distinct from the receptor for EGF and the authors conclude that TGF-..beta.. stimulation of 2-deoxyglucose uptake requires the co-activation of the EGF receptor kinase system.
Research Organization:
Vanderbilt Univ., School of Medicine, Nashville, TN
OSTI ID:
6301199
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 82:5; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANIMAL GROWTH
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL TRANSFORMATIONS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FIBROBLASTS
GLUCOSE
GROWTH
HEXOSES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MATERIALS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RECEPTORS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE