Regulation of translocated c-myc genes transfected into plasmacytoma cells
The authors have transfected two translocated c-myc oncogene clones, derived from two human lymphomas carrying the t(8;14) chromosome translocation, into mouse plasmacytoma cells to study the regulation of their expression. In one case, the transfected clone contained the two coding exons of the c-myc oncogene translocated to an immunoglobulin heavy-chain switch region; in the other case, the two coding exons were translocated 5' of the enhancer element located between the heavy-chain joining region (J/sub H/) and the switch region S/sub ..mu../. Nuclease S1 protection experiments indicate that only the c-myc translocated 5' of the enhancer element is transcribed in the plasmacytoma cells. Thus, 5'-truncation of the c-myc gene per se does not lead to c-myc deregulation. Further, since the level of c-myc transcripts in the parental human lymphoma cells was 3- to 4-fold higher than in the transfectants, it seems likely that additional elements within the heavy-chain locus may play a role in the enhancement of c-myc gene transcription in lymphoma cells.
- Research Organization:
- Wistar Institute, Philadelphia, PA
- OSTI ID:
- 6470210
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ONCOGENES
GENE REGULATION
PHOSPHORUS 32
TRACER TECHNIQUES
BACTERIOPHAGES
ELECTROPHORESIS
GENETIC MAPPING
HYBRIDIZATION
IMMUNOGLOBULINS
MAN
MICE
NUCLEASES
NUCLEOTIDES
RECOMBINANT DNA
TRANSCRIPTION
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DNA
ENZYMES
ESTERASES
GENES
GLOBULINS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAPPING
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RODENTS
VERTEBRATES
VIRUSES
550201* - Biochemistry- Tracer Techniques