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Sequence analysis of the MYC oncogene involved in the t(8; 14)(q24; q11) chromosome translocation in a human leukemia T-cell line indicates that putative regulatory regions are not altered

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ; ; ; ;
The authors cloned the translocation-associated and homologous normal MYC alleles from SKW-3, a leukemia T-cell line with the t(8; 14)(q24; q11) translocation, and determined the sequence of the MYC oncogene first exon and flanking 5' putative regulatory regions. S1 nuclease protection experiments utilizing a MYC first exon probe demonstrated transcriptional deregulation of the MYC gene associated with the T-cell receptor ..cap alpha.. locus on the 8q + chromosome of SKW-3 cells. Nucleotide sequence analysis of the translocation-associated (8q +) MYC allele identified a single base substitution within the upstream flanking region; the homologous nontranslocated allele contained an additional substitution and a two-base deletion. None of the deletions or substitutions localized to putative 5' regulatory regions. The MYC first exon sequence was germ line in both alleles. These results demonstrate that alterations within the putative 5' MYC regulatory regions are not necessarily involved in MYC deregulation in T-cell leukemias, and they show that juxtaposition of the T-cell receptor ..cap alpha.. locus to a germ-line MYC oncogene results in MYC deregulation.
Research Organization:
Wistar Institute, Philadelphia, PA (USA)
OSTI ID:
6142422
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:9; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CLONING
DAYS LIVING RADIOISOTOPES
DISEASES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENE REGULATION
GENES
GENETIC MAPPING
GENETICS
HEMIC DISEASES
HETEROCHROMOSOMES
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
ISOTOPES
KARYOTYPE
LEUKEMIA
LIGHT NUCLEI
MAMMALS
MAN
MAPPING
MUTATIONS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ONCOGENES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TUMOR CELLS
VERTEBRATES