Effects of ethylene on gene expression in carrot roots

Thesis/Dissertation · Sun Jan 01 00:00:00 EST 1984

OSTI ID:6400964

Nichols, S E

To investigate ethylene effects on expression of genetic information, cDNA clones corresponding to ethylene-induced carrot root mRNAs were constructed and isolated. RNA dot blot analysis showed that for the three clones studied peak cytosolic mRNA prevalence occurred at 21 hours of treatment followed thereafter by rapid messenger decay. DNA filter excess hybridization to in vitro synthesized nuclear RNA showed that the ethylene-induced mRNA increase is engendered by transcription of previously quiescent genes. The kinetics and magnitude of changes in mRNA prevalence parallel changes in transcriptional activity; therefore, the ethylene effect is primarily at the level of the transcription. In vivo pulse labelling with (/sup 35/S)-methionine showed that between 18 and 27 hours of ethylene treatment a 2.5 fold increase in translational efficiency occurred for one message studied. The resulting protein is the predominant protein synthesized in carrots treated with ethylene for 27 hours. Thus, ethylene exerts multiple regulatory controls on the expression of genetic information.

• Research Organization: California Univ., Los Angeles (USA)
• OSTI ID: 6400964
• Resource Relation: Other Information: Thesis (Ph. D)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CARROTS
DNA-CLONING
ETHYLENE
BIOLOGICAL EFFECTS
GENES
TRANSCRIPTION
DNA
MESSENGER-RNA
METHIONINE
PROTEINS
ROOTS
SULFUR 35
TRACER TECHNIQUES
ALKENES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CLONING
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FOOD
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIOISOTOPES
RNA
SULFUR ISOTOPES
VEGETABLES
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques