Identification and sequencing of members of a drought-induced multigene family in Atriplex canescens (salt bush)

Conference · Wed May 01 04:00:00 EDT 1991 · Plant Physiology, Supplement; (United States)

OSTI ID:5403448

Chen, Jing; Cairney, J; Newton, R J; Funkhouser, E A ^[1]

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1. Texas A and M Univ., College Station (United States)

Atriplex canescens (Pursh.) Nutt. is known to have a high degree of morphological and physiological drought-tolerance, which appears to be related to molecular responses. A cDNA library, constructed from drought-induced messenger RNA, was differentially screened with radioactively labelled cDNA probes synthesized from mRNA extracted from stressed and non-stressed Atriplex. Two clones named 19-3 and 27-3, whose expression is induced by drought-stress, have been characterized. Sequence analysis shows that they are more than 96% homologous. Each clone has an open reading frame which specifies a protein of 95 amino acids (12.77 kDa and 12.74 kDa respectively.) In vitro transcription and translation of each clone results in a single protein of apparent molecular weight 8.6 kDa. The disparity in size may be due to secondary structure, dictated, at least in part, by a highly charged carboxy terminus which may be important for the function of these proteins in drought tolerance.

• OSTI ID: 5403448
• Report Number(s): CONF-9107184--
• Conference Information: Journal Name: Plant Physiology, Supplement; (United States) Journal Volume: 96:1
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540210 -- Environment
Terrestrial-- Basic Studies-- (1990-)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL STRESS
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DROUGHTS
ENVIRONMENTAL EFFECTS
GENES
HYBRIDIZATION
MESSENGER-RNA
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RNA
SHRUBS
STRUCTURAL CHEMICAL ANALYSIS
TOLERANCE
TRANSCRIPTION