Canine pancreas and kidney transplantation following total-lymphoid irradiation

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Transplantation; (USA)

DOI:https://doi.org/10.1097/00007890-199010000-00009· OSTI ID:6270811

Williamson, P; Allen, R D; Deane, S A; Ekberg, H; Grierson, J M; Hawthorne, W J; Mears, D C; Tiver, K; Little, J M; Stewart, G J ^[1]

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1. Westmead Hospital, Sydney, New South Wales (Australia)

The effect of total-lymphoid irradiation on survival of canine pancreas and kidney allografts was studied. TLI had a marked immunosuppressive effect as measured by in vitro immune responses and reduced circulating leukocytes. Despite the changes, median graft survival times for animals treated with 800 cGy (9 days) or 1800 cGy (9.5 days) were not significantly different from untreated control animals (7 days). The addition of low-dose antithymocyte globulin (10 mg/kg/day) on post-transplant days 0, 2, 4, 6, 8, and 10 had no measurable synergistic effect. Similarly, median segmental pancreas allograft survival times after 1700-2200 cGy of TLI treatment (16.5 days) were only marginally longer than those of untreated controls (9 days). The only animal to maintain a graft for greater than 200 days was matched to the donor in mixed lymphocyte culture (MLC). This animal was able to reject a third-party skin graft after 8 days while a graft from the original donor was still surviving after 21 days when the pancreas graft failed from a chronic-type rejection. These results indicate that TLI alone or in combination with ATG will not be predictably effective as a method of prolonging allograft survival. The role of matching major histocompatibility complex antigens in TLI treatment requires clarification.

• OSTI ID: 6270811
• Journal Information: Transplantation; (USA), Vol. 50:4; ISSN 0041-1337
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
IMMUNITY
RADIOINDUCTION
KIDNEYS
TRANSPLANTS
LYMPHATIC SYSTEM
BIOLOGICAL RADIATION EFFECTS
PANCREAS
DOGS
IMMUNOGLOBULINS
IMMUNOSUPPRESSION
SURVIVAL TIME
ANIMALS
BIOLOGICAL EFFECTS
BODY
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
GLANDS
GLOBULINS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals