Induction of specific unresponsiveness to heart allografts in mongrel dogs treated with total lymphoid irradiation and antithymocyte globulin

Journal Article · Wed Feb 01 04:00:00 EST 1984 · J. Immunol.; (United States)

OSTI ID:5003994

Strober, S; Modry, D L; Hoppe, R T; Pennock, J L; Bieber, C P; Holm, B I; Jamieson, S W; Stinson, E B; Schroder, J; Suomalainen, H; Kaplan, H S

The survival of heterotopic heart allografts was determined in mongrel dogs treated with total lymphoid irradiation (TLI) alone or in combination with other immunosuppressive agents. TLI alone (total dose, 1800 rad) minimally prolonged graft survival as compared with untreated controls. However, marked synergy was observed when TLI was combined with a 10-day post-transplant course of rabbit anti-dog thymocyte globulin (ATG). Approximately 40% of recipients given TLI and ATG showed specific unresponsiveness, as judged by the lack of rejection on serial biopsies for more than 1 year and the prompt rejection of third party hearts. The addition of post-transplant azathioprine (90 to 180 days) to the TLI and ATG regimen increased the mortality of recipients and reduced the fraction of dogs showing specific unresponsiveness. Infusion of donor bone marrow cells at the time of heart transplantation failed to induced specific unresponsiveness in recipients given TLI alone or TLI in combination with post-transplant methotrexate, cyclosporine A, or ATG. The results indicate that the combination of TLI and a brief course of ATG without marrow transplantation was the most effective regimen for the induction of specific unresponsiveness in mongrel dogs.

• Research Organization: Stanford Univ. School of Medicine, CA
• OSTI ID: 5003994
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 132:2; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARDIOVASCULAR SYSTEM
DOGS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
GLOBULINS
GRAFTS
HEART
IMMUNOGLOBULINS
IMMUNOSUPPRESSION
IONIZING RADIATIONS
IRRADIATION
LYMPHATIC SYSTEM
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PARTIAL BODY IRRADIATION
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
TRANSPLANTS
VERTEBRATES
X RADIATION