Synergistic effects of combined immunosuppressive modulation. I. Unresponsiveness to dendritic cell-depleted renal allografts in dogs exposed to total-lymphoid irradiation

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Transplantation; (United States)

OSTI ID:7195043

Rapaport, F T; Meek, A; Miura, S; Hayashi, R; Arnold, A N; Strober, S

Attenuation of the allogeneic stimulus provided by dendritic cells (DC) was achieved by irradiation of the donors, followed by their reconstitution with bone marrow from the prospective DLA-identical recipient. Following long-term (131-187 days) recovery free of graft-versus-host (GVH) disease, the chimeric kidneys were placed into the corresponding recipients; such allografts were rejected at 55, 55, and 60 days, respectively. Four other recipients were conditioned with 1750-1790 cgy of total lymphoid irradiation (TLI) and were then given a similar chimeric kidney from the corresponding partner. These allografts currently survive for 296, 295, 290, and 252 days, respectively. A third group of four dogs was exposed to TLI prior to transplantation of a normal DLA-identical kidney. These grafts were rejected at 20, 42, 46, and 242 days, respectively. Thirteen DLA-identical renal allografts transplanted into normal dogs survived for 13-38 days (mean survival time = 28.6 days). Depletion of allogeneic DC alone, or TLI alone, produced relative prolongations in allograft survival in canine recipients. Combined use of these two modalities, however, resulted in long-term allogeneic unresponsiveness in the recipients.

• Research Organization: State Univ. of New York, Stony Brook (USA)
• OSTI ID: 7195043
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 45:4; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BODY
BONE MARROW
CHIMERAS
DOGS
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
IMMUNOSUPPRESSION
IRRADIATION
KIDNEYS
LYMPHATIC SYSTEM
MAMMALS
MOSAICISM
ORGANS
RADIATION CHIMERAS
SURVIVAL TIME
SYNERGISM
TISSUES
TRANSPLANTS
VERTEBRATES