Characterization of embryo-specific genes

Technical Report · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6171067

Sung, Z R

The objective of the proposed research is to characterize the structure and function of a set of genes whose expression is regulated in embryo development, and that are not expressed in mature tissues -- the embryogenic genes. In order to isolate these genes, we immunized a rabbit with total extracts of somatic embryos of carrot, and enriched the anti-embryo antiserum for antibodies reacting with extracts of carrot somatic embryos. Using this enriched antiserum, we screened a lambda gt11 cDNA library constructed from embryo poly A{sup +} RNA, and isolated 10 cDNA clones that detect embryogenic mRNAs. Monospecific antibodies have been purified for proteins corresponding to each cDNA sequence. Four cDNA clones were further characterized in terms of the expression of their corresponding mRNA and protein in somatic embryos of carrot. In some cases, comparable gene sequences or products have been detected in somatic and zygotic embryos of other plant species. The characteristics of these 4 cDNA clones -- clone Nos. 8, 59, and 66 -- are described in this report. 3 figs.

• Research Organization: California Univ., Berkeley, CA (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: FG03-87ER13698
• OSTI ID: 6171067
• Report Number(s): DOE/ER/13698-T1; ON: DE91006870
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARROTS
DNA-CLONING
EMBRYONIC CELLS
GENE REGULATION
ANTIBODIES
DNA SEQUENCING
EMBRYOS
GENETIC MAPPING
IMMUNE SERUMS
MESSENGER-RNA
PROGRESS REPORT
PROTEINS
PURIFICATION
ANIMAL CELLS
CLONING
DNA HYBRIDIZATION
DOCUMENT TYPES
FOOD
HYBRIDIZATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RNA
STRUCTURAL CHEMICAL ANALYSIS
VEGETABLES
550400* - Genetics
550200 - Biochemistry