Characterization of embryo-specific genes. Final report, April 1, 1987--March 31, 1992
The objective of the proposed research is to characterize the function and regulation of a set of embryonic genes which are expressed in the embryos, not in the plants. 22 cDNA clones were isolated from a cDNA library we constructed using mRNAS of -carrot somatic embryos. These cDNA clones identified mRNA species that are present in the somatic and zygotic embryos, but not in adult plants. The sequence of all 22cDNA clones were determined; genomic clones for three cDNA clones, DC8, DC59, and DC49 were isolated and gene sequences determined. DC8, DC49, and several other genes identified by the cDNA sequences belong to the category of late embryogenesis abundant protein genes, Lea. The function of these gens have not yet been determined, but they share common structural features, are regulated by ABA and are speculated to play a role in seed desiccation.
- Research Organization:
- California Univ., Berkeley, CA (United States). Dept. of Plant Biology
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG03-87ER13698
- OSTI ID:
- 10153749
- Report Number(s):
- DOE/ER/13698--T3; ON: DE92016151
- Country of Publication:
- United States
- Language:
- English
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