Characterization of embryo-specific genes

Technical Report · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6072092

The objective of the proposed research is to characterize the structure and function of a set of genes whose expression is regulated in embryo development, and that is not expressed in mature tissues -- the embryonic genes. In the last two years, using cDNA clones, we have isolated 22 cDNA clones, and characterized the expression pattern of their corresponding RNA. At least 4 cDNA clones detect RNAs of embryonic genes. These cDNA clones detect RNAs expressed in somatic as well as zygotic embryos of carrot. Using the cDNA clones, we screened the genomic library of carrot embryo DNA, and isolated genomic clones for three genes. The structure and function of two genes DC 8 and DC 59 have been characterized and are reported in this paper.

• Research Organization: California Univ., Berkeley, CA (USA). Dept. of Physics
• Sponsoring Organization: DOE/ER
• DOE Contract Number: FG03-87ER13698
• OSTI ID: 6072092
• Report Number(s): DOE/ER/13698-T2; ON: DE91006869
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CARROTS
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
EMBRYONIC CELLS
EMBRYOS
FOOD
GENE REGULATION
HYBRIDIZATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
PROGRESS REPORT
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
VEGETABLES