Increase of translatable mRNA for major microsomal proteins in n-alkane-grown Candida maltosa

Journal Article · Sat Dec 01 00:00:00 EST 1984 · J. Bacteriol.; (United States)

OSTI ID:6053986

Sunairi, M; Watabe, K; Takagi, M; Yano, K

In an n-alkane-assimilating Candida sp., transfer from glucose- to n-alkane-containing medium induced changes in the microsomal proteins, and several distinctive polypeptides were demonstrated in the solubilized microsomal fraction derived from n-alkane-grown cells. Long-term-labeling and pulse-labeling experiments in vivo demonstrated the synthesis of the specific microsomal polypeptides. The polypeptides were synthesized as in vitro translation products directed by polyadenylated RNA extracted from n-alkane-grown cells. Two major polypeptides were partially purified from the microsomal fraction from n-alkane-grown cells, and antiserum was prepared in a rabbit. Immunoprecipitation of these two polypeptides was accompanied by an increase in the amount of translatable mRNA. The molecular weights of the polypeptides derived from long-term-labeling, pulse-labeling and in vitro translation experiments appeared to be identical.

• Research Organization: Univ. of Tokyo, Japan
• OSTI ID: 6053986
• Journal Information: J. Bacteriol.; (United States), Vol. 160:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
BIOLOGICAL EFFECTS
POLYPEPTIDES
BIOSYNTHESIS
CANDIDA
CULTURE MEDIA
IMMUNE SERUMS
LABELLING
MESSENGER-RNA
MICROSOMES
RABBITS
RNA
ANIMALS
CELL CONSTITUENTS
FUNGI
HYDROCARBONS
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PEPTIDES
PLANTS
PROTEINS
SYNTHESIS
VERTEBRATES
YEASTS
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)