Native size of protein phosphatase C-I is 38 kDa as determined by immunoprecipitation of the in vitro translation products of total rabbit muscle mRNA

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6832450

Zheng, S Y; Lee, E Y.C.

PolyA/sup +/ mRNA was prepared from rabbit skeletal muscle. The mRNA was translated in vitro using a rabbit reticulocyte lysate system; the in vitro translation products were labelled using /sup 35/-methionine. The products were immunoprecipitated using a monoclonal antibody directed against phosphatase C-I. This enzyme preparation had previously been observed to have a molecular weight of 32 kDa. The immunoprecipitated products were examined by autoradiography after SDS-PAGE. The major immunoprecipitated polypeptides were of 38 kDa and 35 kDa. These findings indicate that the native size of phosphatase C-I is 38 kDa. These results are consistent with the finding in this and other laboratories that if the enzyme preparation is performed in the presence of protease inhibitors, a 38 kDa enzyme, rather than a 32 kDa enzyme is obtained.

• Research Organization: Univ. of Miami School of Medicine, FL
• OSTI ID: 6832450
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIBODIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENZYME INHIBITORS
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
HYDROLASES
IN VITRO
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MESSENGER-RNA
METHIONINE
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHATASES
RADIOISOTOPES
RNA
SULFUR 35
SULFUR ISOTOPES