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Characterization of antigens from Schistosoma mansoni and construction of a cDNA library for the study of schistosomiasis

Thesis/Dissertation ·
OSTI ID:6550460

To examine the antigens of adult Schistosoma mansoni, /sup 35/S-methionine-labelled, detergent-extracted proteins were immunoprecipitated and analyzed on SDS-PAGE. Human infection serum immunoprecipitated 14 polypeptides with M/sub r/'s of 120, 105, 88, 86, 66, 64, 54, 48, 42, 38, 35, 32, 29, and 20 Kd. Upon digestion with endoglycosidase F polypeptides with M/sub r/'s of 120, 105, 54, 48, and 29 appeared to have carbohydrate moieties. Extracts of female and male S. mansoni were analyzed by immunoprecipitation and immunoblotting. Two polypeptides with M/sub r/'s of 86 Kd and 54 Kd were detected only in extracts of males. Polyadenylated RNA was extracted from S. mansoni and translated in rabbit reticulocyte lysates. Among the in vitro translation products, polypeptides with 120, 94, 64, 43, 37, 35, 30, 26 and 22 Kd apparent molecular weights were immunoprecipitated by human infection serum. When the translation products of female worms and male worms were compared, the polypeptides with M/sub r/'s of 94 and 64 Kd were only observed in males.

Research Organization:
California Univ., Los Angeles (USA)
OSTI ID:
6550460
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
CLONING
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA-CLONING
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
HELMINTHS
IMMUNE REACTIONS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
MOLECULAR WEIGHT
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITIC DISEASES
PATHOGENESIS
PEPTIDES
PLATYHELMINTHS
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RNA
SCHISTOSOMA
SCHISTOSOMIASIS
SEX DEPENDENCE
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TREMATODES