Identification of proteins specific for human herpesvirus 6-infected human T cells
Proteins specific for human herpesvirus 6 (HHV-6)-infected human T cells (HSB-2) were examined by using polyclonal rabbit antibodies and monoclonal antibodies against HHV-6-infected cells and human sera. More than 20 proteins and six glycoproteins specific for HHV-6-infected cells were identified from (/sup 35/S)methionine- and (/sup 3/H)glucosamine-labeled total-cell extracts. Polyclonal rabbit antibodies immunoprecipitated 33 (/sup 35/S)methionine-labeled HHV-6-specific polypeptides with approximate molecular weights ranging from 180,000 to 31,000. In immunoprecipitation and Western immunoblot reactions, a patient's serum also recognized more than 30 HHV-6-specific proteins and seven glycoproteins. In contrast, sera from individuals with high-titered antibodies against other human herpes viruses reacted with few HHV-6-infected cell proteins, and only a 135,000-M/sub r/ polypeptide was prominent. Monoclonal antibodies to HHV-6-infected cells reacted with single and multiple polypeptides specific for virus-infected cells and immunoprecipitated three distinct sets of glycoproteins, which were designated gp105K and gp92k, gp116k, gp64k, and gp54k, and gp102k.
- Research Organization:
- Univ. of Kansas Medical Center, Kansas City (USA)
- OSTI ID:
- 5991583
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 63:62; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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