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cDNA cloning and expression of a human platelet-derived growth factor (PDGF) receptor specific for B-chain-containing PDGF molecules

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.8.8.3476· OSTI ID:7095610
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The structure of the human receptor for platelet-derived growth factor (PDGF) has been deduced through cDNA cloning. A 5.45-kilobase-pair cDNA clone predicts a 1,106-amino-acid polypeptide, including the cleavable signal sequence. The overall amino acid sequence similarity with the murine PDGFR receptor is 85%. After transcription of the cDNA and translation in vitro, a PDGR receptor antiserum was used to immunoprecipitate a product of predicted size, which also could be phosphorylated in vitro. Stable introduction of the cDNA into Chinese hamster ovary (CHO) cells led to the expression of a 190-kilodalton component, which was immunoprecipitated by the PDGF receptor antiserum; this most probably represents the mature PDGF receptor. Binding assays with different /sup 125/I-labeled dimeric forms of PDGF A and B chains showed that the PDGFR receptor expressed in CHO cells bound PDGF-BB and, to a lesser extent, PDGF-AB, but not PDGF-AA.

Research Organization:
Ludwig Institute for Cancer Research, Uppsala Branch, Box 595, Biomedical Center, S-751 23 Uppsala (SE)
OSTI ID:
7095610
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:8; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CHO CELLS
CLONING
DATA
DAYS LIVING RADIOISOTOPES
DIMERS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GROWTH FACTORS
HYBRIDIZATION
IMMUNOLOGY
IN VITRO
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLING
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHENOTYPE
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
RODENTS
SPECIES DIVERSITY
SPECIFICITY
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES