cDNA cloning and expression of a human platelet-derived growth factor (PDGF) receptor specific for B-chain-containing PDGF molecules

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.8.3476· OSTI ID:7095610

Claesson-Welsh, L; Eriksson, A; Moren, A; Severinsson, L; Ek, B; Ostman, A; Betsholtz, C; Heldin, C H

The structure of the human receptor for platelet-derived growth factor (PDGF) has been deduced through cDNA cloning. A 5.45-kilobase-pair cDNA clone predicts a 1,106-amino-acid polypeptide, including the cleavable signal sequence. The overall amino acid sequence similarity with the murine PDGFR receptor is 85%. After transcription of the cDNA and translation in vitro, a PDGR receptor antiserum was used to immunoprecipitate a product of predicted size, which also could be phosphorylated in vitro. Stable introduction of the cDNA into Chinese hamster ovary (CHO) cells led to the expression of a 190-kilodalton component, which was immunoprecipitated by the PDGF receptor antiserum; this most probably represents the mature PDGF receptor. Binding assays with different /sup 125/I-labeled dimeric forms of PDGF A and B chains showed that the PDGFR receptor expressed in CHO cells bound PDGF-BB and, to a lesser extent, PDGF-AB, but not PDGF-AA.

• Research Organization: Ludwig Institute for Cancer Research, Uppsala Branch, Box 595, Biomedical Center, S-751 23 Uppsala (SE)
• OSTI ID: 7095610
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 8:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
RECEPTORS
DNA SEQUENCING
DNA-CLONING
AMINO ACID SEQUENCE
BLOOD PLATELETS
CHO CELLS
DIMERS
EXPERIMENTAL DATA
IMMUNOLOGY
IN VITRO
IODINE 125
LABELLING
MAN
MICE
MOLECULAR STRUCTURE
PHENOTYPE
POLYPEPTIDES
SPECIES DIVERSITY
SPECIFICITY
TRANSCRIPTION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
DATA
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
ELECTRON CAPTURE RADIOISOTOPES
HYBRIDIZATION
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MITOGENS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques