Mucosal iron in the control of iron absorption in a rat intestinal transplant model

Journal Article · Fri Feb 01 00:00:00 EST 1991 · Gastroenterology; (USA)

OSTI ID:5988034

Adams, P C; Zhong, R; Haist, J; Flanagan, P R; Grant, D R ^[1]

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1. Univ. of Western Ontario, London (Canada)

Isogeneic intestinal transplantation of iron-loaded and iron-deficient intestine into iron-deficient rats was performed in 20 Lewis rats to isolate the effect of intestinal mucosal iron on iron absorption. Rats were iron loaded with three weekly IM injections of 50 mg of iron dextran and were rendered iron deficient with an iron-deficient diet for 3 weeks. Iron status was assessed by hepatic and gut mucosal iron determination. Uptake and transfer of 59Fe-ascorbate was measured in an isolated perfused segment of transplanted intestine 48 hours after transplantation. The mean rate of uptake of 59Fe from an iron-loaded intestine (mean mucosal iron concentration, 7.97 +/- 2.02 mumol/g) was 431 +/- 27 nmol/30 min, and from an iron-deficient intestine (mean mucosal iron concentration, 1.35 +/- .84 mumol/g), 743 +/- 222 nmol/30 min (P less than 0.001). The mean transfer of 59Fe from the mucosal cell to the body through an iron-loaded intestine was 63 +/- 22 nmol/30 min, and through an iron-deficient intestine was 86 +/- 32 nmol/30 min (P less than 0.05). These results suggest that the gut mucosal iron concentration regulates the uptake and transfer of iron in the intestine.

• OSTI ID: 5988034
• Journal Information: Gastroenterology; (USA), Vol. 100:2; ISSN 0016-5085
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IRON
INTESTINAL ABSORPTION
SMALL INTESTINE
TRANSPLANTS
BIOLOGICAL MODELS
IRON 59
METABOLISM
MUCOUS MEMBRANES
NUTRITIONAL DEFICIENCY
RATS
TRACER TECHNIQUES
ABSORPTION
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELEMENTS
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINES
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
METALS
NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
TRANSITION ELEMENTS
UPTAKE
VERTEBRATES
550501* - Metabolism- Tracer Techniques