Exposure of surfactant protein A to ozone in vitro and in vivo impairs its interactions with alveolar cells

Journal Article · Wed Jan 01 00:00:00 EST 1992 · American Journal of Physiology; (United States)

OSTI ID:5905622

Oosting, R S; Van Iwaarden, J F; Van Bree, L; Verhoef, J; Van Golde, L M; Haagsman, H P ^[1]

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1. Laboratory of Veterinary Biochemistry, Utrecht University (Netherlands)

This study focused on the question of whether exposure of surfactant protein A (SP-A) to ozone affected properties of this protein that may be involved in regulating alveolar type II cell and alveolar macrophage functions. In vitro exposure of human or canine SP-A to ozone reduced the ability of this protein to inhibit phorbol-ester induced secretion of (3H)phosphatidylcholine by alveolar type II cells in culture. Ozone-exposed human SP-A showed a decreased ability to enhance phagocytosis of herpes simplex virus and to stimulate superoxide anion production by alveolar macrophages. Experiments with elastase showed that ozone-exposed canine SP-A was more susceptible to proteolysis. A conformational change of the protein could underlie this phenomenon. Surfactant isolated from ozone-exposed rats (0.4 ppm ozone for 12 h) was also less able to stimulate superoxide anion production by alveolar macrophages than surfactant from control rats, which suggested that SP-A in vivo was also susceptible to ozone. The results of this study suggest that SP-A-alveolar cell interactions can be inhibited by ozone exposure, which may contribute to the toxicity of ozone in the lungs.

• OSTI ID: 5905622
• Journal Information: American Journal of Physiology; (United States), Vol. 262:1 Pt 1; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LUNGS
SENSITIVITY
OZONE
TOXICITY
PHORBOL ESTERS
BIOLOGICAL EFFECTS
PROTEINS
CHEMICAL PROPERTIES
DOGS
IN VITRO
IN VIVO
LAVAGE
MACROPHAGES
MAN
PHAGOCYTOSIS
PHOSPHOLIPIDS
RATS
TRYPAN BLUE
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZO COMPOUNDS
AZO DYES
BODY
CARCINOGENS
CONNECTIVE TISSUE CELLS
DYES
ESTERS
HYDROXY COMPOUNDS
LIPIDS
MAMMALS
NAPHTHOLS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHAGOCYTES
PHENOLS
PRIMATES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SULFONIC ACIDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology