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Title: Exposure of surfactant protein A to ozone in vitro and in vivo impairs its interactions with alveolar cells

This study focused on the question of whether exposure of surfactant protein A (SP-A) to ozone affected properties of this protein that may be involved in regulating alveolar type II cell and alveolar macrophage functions. In vitro exposure of human or canine SP-A to ozone reduced the ability of this protein to inhibit phorbol-ester induced secretion of (3H)phosphatidylcholine by alveolar type II cells in culture. Ozone-exposed human SP-A showed a decreased ability to enhance phagocytosis of herpes simplex virus and to stimulate superoxide anion production by alveolar macrophages. Experiments with elastase showed that ozone-exposed canine SP-A was more susceptible to proteolysis. A conformational change of the protein could underlie this phenomenon. Surfactant isolated from ozone-exposed rats (0.4 ppm ozone for 12 h) was also less able to stimulate superoxide anion production by alveolar macrophages than surfactant from control rats, which suggested that SP-A in vivo was also susceptible to ozone. The results of this study suggest that SP-A-alveolar cell interactions can be inhibited by ozone exposure, which may contribute to the toxicity of ozone in the lungs.
Authors:
; ; ; ; ;  [1]
  1. (Laboratory of Veterinary Biochemistry, Utrecht University (Netherlands))
Publication Date:
OSTI Identifier:
5905622
Resource Type:
Journal Article
Resource Relation:
Journal Name: American Journal of Physiology; (United States); Journal Volume: 262:1 Pt 1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LUNGS; SENSITIVITY; OZONE; TOXICITY; PHORBOL ESTERS; BIOLOGICAL EFFECTS; PROTEINS; CHEMICAL PROPERTIES; DOGS; IN VITRO; IN VIVO; LAVAGE; MACROPHAGES; MAN; PHAGOCYTOSIS; PHOSPHOLIPIDS; RATS; TRYPAN BLUE; AMINES; ANIMAL CELLS; ANIMALS; AROMATICS; AZO COMPOUNDS; AZO DYES; BODY; CARCINOGENS; CONNECTIVE TISSUE CELLS; DYES; ESTERS; HYDROXY COMPOUNDS; LIPIDS; MAMMALS; NAPHTHOLS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; PHAGOCYTES; PHENOLS; PRIMATES; RESPIRATORY SYSTEM; RODENTS; SOMATIC CELLS; SULFONIC ACIDS; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology