Modulation of human alveolar macrophage properties by ozone exposure in vitro
- ABB Environmental Services, Inc., Chapel Hill, NC (United States)
The authors have investigated changes in human alveolar macrophage (HAM) function after exposure in vitro to ozone (O3). The functions studied reflect concern that O3 is detrimental to host defense mechanisms in the bronchoalveolar spaces. Exposure of HAM to O3 caused a concentration-dependent increase in release of prostaglandin E2 (PGE2), an important modulator of inflammation, phagocytosis, and oxidative burst. Although phagocytosis of particulate immune complexes was decreased by O3, we found no change in the quantity of Fc receptors and complement receptors on the HAM surface. Superoxide (O2-) production in response to phorbol ester was reduced after exposure of HAM to O3 while the basal O2- release in response to plastic adherence was not affected. Growth inhibition of the opportunistic yeast Cryptococcus neoformans by HAM was not affected by O3 exposure. The production of inflammatory mediators and immune modulators such as tumor necrosis factor-alpha, interleukin 1, and interleukin 6 were not induced by exposure to O3. However, compared to controls, O3- exposed HAM produced significantly lower levels of these cytokines when stimulated with bacterial lipopolysaccharide (LPS). Two-dimensional gel electrophoretic analysis of proteins made by HAM following in vitro exposure to O3 identified 11 proteins whose rate of synthesis was significantly altered. Thus, these studies show that exposure to O3 alters the functional competence of HAM. While there is a minimal effect on protein expression or synthesis, the responses of HAM to particulate immune complexes, to bacterial LPS, and to PMA are impaired. The release of arachidonic acid and PGE2 suggest that the effect of O3 is primarily targeted to the HAM cell membrane. These changes may ultimately result in increased susceptibility to inhaled infectious agents in the O3-exposed individual.
- OSTI ID:
- 6097302
- Journal Information:
- Toxicology and Applied Pharmacology; (United States), Vol. 110:3; ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Exposure of humans to ambient levels of ozone for 6. 6 hours causes cellular and biochemical changes in the lung
Cryptococcus neoformans - Infected Macrophages Release Proinflammatory Extracellular Vesicles: Insight into Their Components by Multi-omics
Related Subjects
MACROPHAGES
SENSITIVITY
OZONE
TOXICITY
PHORBOL ESTERS
BIOLOGICAL EFFECTS
BRONCHI
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
LYMPHOKINES
MAN
PROTEINS
RECEPTORS
TWO-DIMENSIONAL ELECTROPHORESIS
YEASTS
ANIMAL CELLS
ANIMALS
CARCINOGENS
CONNECTIVE TISSUE CELLS
ELECTROPHORESIS
ESTERS
EUMYCOTA
FUNGI
GROWTH FACTORS
MAMMALS
MEMBRANE PROTEINS
MICROORGANISMS
MITOGENS
ORGANIC COMPOUNDS
PHAGOCYTES
PLANTS
PRIMATES
RESPIRATORY SYSTEM
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology