Graft-versus-host reaction and immune function. III. Functional pre-T cells in the bone marrow of graft-versus-host-reactive mice displaying T cell immunodeficiency
Studies were performed to determine whether pre-T cells develop normally in the bone marrow of mice displaying thymic dysplasia and T cell immunodeficiency as a consequence of a graft-versus-host (GVH) reaction. GVH reactions were induced in CBAxAF1 mice by the injection of A strain lymphoid cells. To test for the presence of pre-T cells in GVH-reactive mice, bone marrow from GVH-reactive mice (GVHBM) was injected into irradiated syngeneic F1 mice and 30-40 days later thymic morphology and function were studied. Morphology studies showed nearly normal thymic architectural restoration; moreover, such glands contained normal numbers of Thy-1-positive cells. Functional pre-T cells were evaluated by transferring thymocytes from the irradiated GVHBM-reconstituted mice into T-cell-deprived mice. These thymocytes reconstituted allograft reactivity, T helper cell function and Con A and PHA mitogen responses of T-cell-deprived mice. These results suggest that the pre-T cell population in the bone marrow is not affected by the GVH reaction. Therefore, the T cell immunodeficiency associated with the GVH reaction is not due to a deficiency of pre-T cells in the bone marrow but is more likely associated with GVH-induced thymic dysplasia.
- Research Organization:
- McGill Univ., Montreal, Quebec, Canada
- OSTI ID:
- 5859455
- Journal Information:
- Transplantation; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
THYMUS
MORPHOLOGICAL CHANGES
TRANSPLANTS
IMMUNE REACTIONS
ANTIBODY FORMATION
BIOLOGICAL RADIATION EFFECTS
BONE MARROW
GRAFT-HOST REACTION
IMMUNOSUPPRESSION
LYMPHOCYTES
MICE
MORPHOLOGY
RADIOINDUCTION
THYMOCYTES
THYMUS CELLS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
HEMATOPOIETIC SYSTEM
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
ORGANS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals