Graft-versus-host reaction and immune function. IV. B cell functional defect associated with a depletion of splenic colony-forming units in marrow of graft-versus-host-reactive mice

Journal Article · Sat Feb 01 04:00:00 EST 1986 · Transplantation; (United States)

OSTI ID:5633011

Seddik, M; Seemayer, T A; Lapp, W S

Studies were conducted to determine whether a functional B cell defect occurred in the bone marrow of mice experiencing a GVH reaction (GVHBM). GVH reactions were induced in AxCBA F1 adult mice by an injection of A strain lymphoid cells. The GVH reaction was confirmed by immunosuppression and thymus histology. At various intervals after GVH induction, GVHBM was tested for its ability to restore B cell function in adult thymectomized irradiated mice reconstituted with normal thymocytes. GVHBM cells obtained seven days after GVH induction restored but slightly the plaque forming cell (PFC) response to sheep erythrocytes and the mitogen response to lipopolysaccharide (LPS). GVHBM cells obtained 10 days or later failed to reconstitute the PFC or LPS responses. GVHBM cells suppressed neither the T or B cell function of normal spleen cells nor the LPS mitogen response of normal bone marrow cells. In addition, the splenic colony-forming units (CFU-s) in GVHBM were slightly decreased by day 10 after GVH induction and markedly depressed by day 22 after GVH induction. These results suggest that the GVH reaction may affect two different events in B cell differentiation. The early decrease in functional B cells that occurs before there is any change in the CFU-s population suggests a direct effect on B cell production, whereas the later absence of functional B cells could be due to the marked decline in stem cell production (CFU-s).

• Research Organization: McGill Univ., Montreal, Quebec, Canada
• OSTI ID: 5633011
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 2; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODY FORMATION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
BONE MARROW CELLS
CFU
CONNECTIVE TISSUE CELLS
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IMMUNOSUPPRESSION
MAMMALS
MICE
ORGANS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
SPLEEN
THYMOCYTES
THYMUS CELLS
TISSUES
TRANSPLANTS
VERTEBRATES