Neutrophil beta-adrenergic receptor responses are potentiated by acute exposure to phorbol ester without changes in receptor distribution or coupling
- Royal College of Surgeons, Dublin (Ireland)
Exposure to the phorbol ester, phorbol 12-myristate, 13-acetate for 10 minutes enhanced cyclic AMP accumulation in human neutrophils under basal conditions and in response to the beta-adrenergic receptor agonist isoproterenol (ISO, 1{mu}M) and the adenylate cyclase activator forskolin (FSK, 10mM). Potentiation of responses to ISO by PMA was dose-dependent between 0.1 and 100nM PMA. The diacylglycerol analogue, 1-oleoyl-2-actylgylcerol (OAG) (50 {mu}M) also elevated beta-receptor responses, but 4beta-phorbol (100nM), lacking the capacity to activate PMA, was ineffective. Short-term exposure to the peptide n-formylmethionine leucyl-phenylalanine (FMLP, 1 {mu}M) also elevated neutrophil cyclic AMP accumulation. All potentiating effects of PMA on cyclic AMP production were inhibited by the protein kinase inhibitor 1-(5-isoquinolinylsulphonyl)-2-methylpiperazine (H{sub 7}). PMA had no apparent effect on beta-receptor agonist-affinity, distribution between cell-surface and internalized compartments, or the capacity of ISO to induce beta-receptor internalization. Responses to FSK or ISO in terms of fold-stimulation of basal cyclic AMP accumulation int he presence of PMA were not elevated by PMA.
- OSTI ID:
- 5751594
- Journal Information:
- Life Sciences; (United States), Vol. 49:10; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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AMP
BIOSYNTHESIS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
SYMPATHOMIMETICS
RECEPTORS
CYCLASES
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYME INHIBITORS
MAN
NEUTROPHILS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
DRUGS
ENZYMES
ESTERS
LEUKOCYTES
LYASES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
NUCLEOTIDES
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology