Induction of desensitization by phorbol ester to. beta. -adrenergic agonist stimulation in adenylate cyclase system of rat reticulocytes
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Treatment of rat reticulocytes with a phorbol ester, tetradecanoyl phorbol acetate (TPA), resulted in the desensitization of adenylate cyclase to the ..beta..-adrenergic agonist stimulation depending on the dose and period of the TPA treatment. Treatment of the reticulocytes with TPA caused approximately 40% reduction in the stimulation by ..beta..-adrenergic agonists of adenylate cyclase activity, whereas the treatment had little effect on the basal activity and the activation by fluoride and guanine nucleotide of the enzyme system. No change in the number of ..beta..-adrenergic receptors was observed after the TPA treatment. Treatment with 1-oleoyl-2-acetyl-glycerol (OAG), an activator of protein kinase C, also caused the desensitization of reticulocyte adenylate cyclase to isoproterenol. On the other hand, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), a potent inhibitor of protein kinase C, prevented the desensitization induced by TPA.
- Research Organization:
- Hiroshima Univ. School of Medicine, Japan
- OSTI ID:
- 6984984
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 138:1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENYLIC ACID
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CHEMICAL ACTIVATION
CYCLASES
DATA
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ERYTHROCYTES
ESTERS
EXPERIMENTAL DATA
FLUORIDES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
INFORMATION
LYASES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RATS
RECEPTORS
RETICULOCYTES
RODENTS
STIMULATION
TRANSFERASES
VERTEBRATES
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENYLIC ACID
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CHEMICAL ACTIVATION
CYCLASES
DATA
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ERYTHROCYTES
ESTERS
EXPERIMENTAL DATA
FLUORIDES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
INFORMATION
LYASES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RATS
RECEPTORS
RETICULOCYTES
RODENTS
STIMULATION
TRANSFERASES
VERTEBRATES