Phorbol esters modulate cyclic AMP accumulation in porcine thyroid cells
Journal Article
·
· Life Sci.; (United States)
In cultured porcine thyroid cells, during 60 min incubation phorbol 12-myristate 13-acetate (PMA) had no effect on basal cyclic AMP accumulation and slightly stimulated cyclic AMP accumulation evoked by thyroid stimulating hormone (TSH) or forskolin. Cholera toxin-induced cyclic AMP accumulation was significantly stimulated by PMA. On the other hand, cyclic AMP accumulation evoked by prostaglandin E/sub 1/ or E/sub 2/ (PGE/sub 1/ and PGE/sub 2/) was markedly depressed by simultaneous addition of PMA. These opposing effects of PMA on cyclic AMP accumulation evoked by PGE and cholera toxin were observed in a dose-related fashion, with half-maximal effect of around 10/sup -9/ M in either case. The almost same effects of PMA on cyclic AMP accumulation in basal and stimulated conditions were also observed in freshly prepared thyroid cells. The present study was performed in the presence of phosphodiesterase inhibitor, 3-iso-butyl-1-methylxanthine (IBMX), indicating that PMA affected adenylate cyclase activity. Therefore, it is suggested that PMA may modulate the production of cyclic AMP in response to different stimuli, possibly by affecting several sites in the adenylate cyclase complex in thyroid cells.
- Research Organization:
- Dokkyo Univ. School of Medicine, Tochigi (Japan)
- OSTI ID:
- 7252535
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 42:22; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
CARCINOGENS
CYCLASES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESTERS
HORMONES
LYASES
MAMMALS
MATERIALS
NUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PITUITARY HORMONES
PROSTAGLANDINS
SWINE
THYROID CELLS
TOXIC MATERIALS
TOXINS
TSH
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
CARCINOGENS
CYCLASES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESTERS
HORMONES
LYASES
MAMMALS
MATERIALS
NUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PITUITARY HORMONES
PROSTAGLANDINS
SWINE
THYROID CELLS
TOXIC MATERIALS
TOXINS
TSH
VERTEBRATES