Evidence that the low-affinity folate-binding protein in erythrocyte hemolysate is identical to hemoglobin
Journal Article
·
· Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:5707773
Gel filtration studies on erythrocyte hemolysate demonstrated the presence of a folate binding protein, apparently of the low-affinity type, that co-elutes with hemoglobin. Further, the folate binder eluted with a low salt concentration after DEAE-Sepharose CL-6B anion-exchange chromatography of erythrocyte hemolysate at pH 6.3. The chromatographic behavior of hemoglobin labeled with (3H)folate was so similar to that of the present binder as to suggest that the folate binder in erythrocytes is in fact hemoglobin.
- OSTI ID:
- 5707773
- Journal Information:
- Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 27:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
FOLIC ACID
BIOCHEMICAL REACTION KINETICS
HEMOGLOBIN
TRITIUM COMPOUNDS
TRACER TECHNIQUES
AFFINITY
BIOCHEMISTRY
ERYTHROCYTES
HEMOLYSIS
PH VALUE
AMINO ACIDS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMISTRY
DRUGS
GLOBIN
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYSIS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PIGMENTS
PORPHYRINS
PROTEINS
PTERIDINES
REACTION KINETICS
VITAMIN B GROUP
VITAMINS
550201* - Biochemistry- Tracer Techniques
FOLIC ACID
BIOCHEMICAL REACTION KINETICS
HEMOGLOBIN
TRITIUM COMPOUNDS
TRACER TECHNIQUES
AFFINITY
BIOCHEMISTRY
ERYTHROCYTES
HEMOLYSIS
PH VALUE
AMINO ACIDS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMISTRY
DRUGS
GLOBIN
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYSIS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PIGMENTS
PORPHYRINS
PROTEINS
PTERIDINES
REACTION KINETICS
VITAMIN B GROUP
VITAMINS
550201* - Biochemistry- Tracer Techniques