Insulin-like substance and insulin-degrading complex of hemolysate of human erythrocytes

Journal Article · Wed Aug 20 00:00:00 EDT 1986 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5726765

Matulyavichyus, V A; Vareikis, E I; Lashas, L V

A lysate of human erythrocytes was fractionated on gel-filtration resins of different types and immunoreactive insulin, the insulinase activity and the effect of individual fractions on the insulinase activity was determined in the fractions obtained. It was established that the hemolysate contains a complex of insulin-metabolizing compounds, including an insulin-like substance, insulinase, and an inhibitor and activator of the insulinase activity. The insulin-like substance coincided with native insulin in site of elution from a column of Sephadex G-50 and its concentration in the lysate exceeded that of insulin in the blood plasma. Insulinase, which has a molecular weight of about 100,000, cleaved (/sup 125/I) insulin to fragments soluble in trichloroacetic acid, but had no effect on hypophyseal proteins and glycoprotein hormones. The insulinase activity was inhibited by low temperatures, atropine, and a newly discovered intraerythrocytic proteinase inhibitor, which also inhibits the serine proteinases trypsin and chymotrypsin. A substance eluted from a column of Sephadex G-100 in the region of low-molecular-weight substances increased the insulinase activity. The elution curve of substances with proteinase-inhibiting and insulinase-activating activities indicates that there is more than one inhibitory and activating factor. The results of the studies suggest that the insulin-degrading complex in human erythrocytes acts as a regulator of the insulin level in the blood plasma. It is also possible that the insulin-like substance is produced in the cytosol of the erythrocytes.

• Research Organization: Institute of Experimental Endocrinology and Hormone Chemistry, Kaunas, USSR
• OSTI ID: 5726765
• Journal Information: Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:2; ISSN BIORA
• Country of Publication: United States
• Language: English

Related Subjects

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550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIODEGRADATION
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLASMA
BODY FLUIDS
CARBOHYDRATES
CHEMICAL REACTIONS
CHEMISTRY
COMPARATIVE EVALUATIONS
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ERYTHROCYTES
FRACTIONATION
GLUCOPROTEINS
HORMONES
HYDROLASES
HYDROLYSIS
IMMUNOASSAY
IMMUNOLOGY
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LYSIS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RADIOASSAY
RADIOENZYMATIC ASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RECEPTORS
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
TRACER TECHNIQUES
VERTEBRATES