Cell-surface proteoglycan in sea urchin primary mesenchyme cell migration

Miscellaneous · Sun Jan 01 00:00:00 EST 1989

OSTI ID:5671379

Lane, M C

Early in the development of the sea urchin embryo, the primary mesenchyme cells (PMC) migrate along the basal lamina of the blastocoel. Migration is inhibited in L. pictus embryos cultured in sulfate-free seawater and in S. purpuratus embryos exposed to exogenous {beta}-D-xylosides. An in vitro assay was developed to test the migratory capacity of normal PMC on normal and treated blastocoelic matrix. Sulfate deprivation and exposure to exogenous xyloside render PMC nonmotile on either matrix. Materials removed from the surface of normal PMC by treatment with 1 M urea restored migratory ability to defective cells, whereas a similar preparation isolated from the surface of epithelial cells at the same stage did not. Migration also resumed when cells were removed from the xyloside or returned to normal seawater. The urea extract was partially purified and characterized by radiolabeling, gel electrophoresis, fluorography, ion exchange chromatography, and western blotting. The PMC synthesize a large chondroitin sulfate/dermatan sulfate proteoglycan that is present in an active fraction isolated by chromatography. Chondroitinase ABC digestion of live cells blocked migration reversibly, further supporting the identification of the chondroitin sulfate/dermatan sulfate proteoglycan as the active component in the urea extract. Much of the incorporated sulfate was distributed along the filopodia in {sup 35}SO{sub 4}-labelled PMC by autoradiography. The morphology of normal and treated S. purpuratus PMC was examined by scanning electron microscopy, and differences in spreading, particularly of the extensive filopodia present on the cells, was observed. A model for the role of the chondroitin sulfate/dermatan sulfate proteoglycan in cell detachment during migration is proposed.

• Research Organization: Iowa Univ., Iowa City, IA (United States)
• OSTI ID: 5671379
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EMBRYONIC CELLS
PHYSIOLOGY
GLYCOPROTEINS
BIOSYNTHESIS
SEA URCHINS
ONTOGENESIS
AUTORADIOGRAPHY
ELECTROPHORESIS
EMBRYOS
GLYCOSYL HYDROLASES
ION EXCHANGE CHROMATOGRAPHY
MATHEMATICAL MODELS
SCANNING ELECTRON MICROSCOPY
SULFATES
SULFUR 35
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ECHINODERMS
ELECTRON MICROSCOPY
ENZYMES
EVEN-ODD NUCLEI
HYDROLASES
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
MICROSCOPY
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques