Developmental expression of a cell surface protein involved in sea urchin skeleton formation. [Strongylocentrotus purpuratus; Lytechinus pictus]
The authors have previously used a monoclonal antibody (1223) to identify a 130 Kd cell surface protein involved in skeleton formation is sea urchin embryos. In the current study the authors have examined the expression of the 1223 antigen over the course of development of embryos of two species, Strongylocentrotus purpuratus and Lytechinus pictus. The 130 Kd protein is detected in S. purp eggs on immunoblots. Labeling with (/sup 3/H) leucine and immunoaffinity chromatography show that it also is synthesized shortly after fertilization. Immunofluroescence reveals that at this early stage the 1223 antigen is uniformly distributed on all of the cells. Synthesis decreases to a minimum by the time of hatching (18 h), as does the total amount of antigen present in the embryo. A second period of synthesis commences at the mesenchyme blastula stage, when the spicule-forming primary mesenchyme cells (PMCs) have appeared. During this later stage, synthesis and cell surface expression are restricted to the PMCs. In contrast to S. purp., in L. pictus the 130 Kd protein does not appear until the PMCs are formed. Hybrid embryos demonstrate a pattern of expression of the maternal species. These results suggest that early expression of 1223 antigen in S. purp. is due to utilization of maternal transcripts present in the egg. In both species later expression in PMCs appears to be the result of cell-type specific synthesis, perhaps encoded by embryonic transcripts.
- Research Organization:
- M.D. Anderson Hospital and Tumor Institute, Houston, TX
- OSTI ID:
- 5269809
- Report Number(s):
- CONF-8606151-; TRN: 86-031543
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
LABELLING
SEA URCHINS
ONTOGENESIS
ANTIGENS
BIOLOGICAL VARIABILITY
CHROMATOGRAPHY
FLUORESCENCE
LEUCINE
SKELETON
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
AQUATIC ORGANISMS
BODY
CARBOXYLIC ACIDS
ECHINODERMS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUMINESCENCE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
SEPARATION PROCESSES
550801* - Morphology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques